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Raising the gold standard for therapeutic protein expression


As market demand continues to rise for more potent and effective therapeutics, drug developers are identifying novel disease targets and biological mechanisms for which medicines can be developed. As a consequence biologic pipelines are evolving from standard antibody formats to next-generation biologics. Now more than ever before, there is a real need for robust and scalable expression platforms that can keep pace with these requirements.

We strive to help you develop next generation biotherapeutics as quickly and reliably as possible. With more than 35 years of experience providing mammalian expression, process development and manufacturing services, we are ideally placed to supply you with a robust and versatile expression platform to optimize your production.

The GS Xceed® Expression System is a market-leading expression technology platform. This robust, fully integrated, scalable system includes host cell lines, vectors, and access to optimized media and feeds, as well as comprehensive methods and processes.

As biopharmaceutical development continues to evolve, we are committed to ongoing technology advancements to our GS Xceed® Toolbox to continue to meet your needs. The combination of this ongoing innovation, advanced, proprietary technology and our exceptional expertise will help improve your efficiency and help reduce time to market.


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Accelerating translation of protein biologics into clinical development: current trends

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Meet the challenge of complex protein expression with GS piggyBac™ 

Introducing the next stage in the evolution of our GS Xceed® Toolbox, GS piggyBac™- a unique and versatile cell line engineering technology.  A proven transposon-based technology that preferentially targets stable regions of the genome associated with highly expressed genes.

The GS piggyBac™ system uses an engineered hyperactive piggyBac™ transposase enzyme to insert GS Xceed® expression vector cargos into the host cell genome with high efficiency with the following benefits:

  • Combining our GS System® with piggyBac™ transposon technology results in increased yields versus GS System® alone with both pools and clones1,2
  • Enhanced performance of GS piggyBac™ is observed with low versus high expressing monoclonal antibodies1,2
  • Accommodating a large DNA cargo (> 200kb) makes GS piggyBac™ highly suited to support cell line engineering needed to enhance complex protein expression3
  • Preferentially targeting genetically stable parts of the genome at high efficiency has the potential to improve cell line stability.4, 5, 6

Available to new and existing GS Expression System® license holders. Contact us to learn more

GS PiggyBac™ - How it Works

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How Lonza’s GS piggyBac™ helps meet the challenge of complex protein expression.

Recent Publications

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Utilising GS piggyBac™ transposon technology to enhance the performance of the Lonza GS Gene Expression System in stable CHO pool construction

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The successful evolution of biotherapeutics from discovery to development

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Tools to meet the challenge of complex protein expression

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Citations:

1Lonza In-house data
2Rajendra et al. (2016), Biotechnology. Progress. 32:1301-1307
3Liet al (2013), Disease Model and Mechanisms 6:828-833
4Rajendra et al. (2017), Biotechnology Progress 33:1436
5Matasci et al. (2011), Biotechnol Bioeng 108:2141
6Mossine et al. (2013), PLoS One

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