Express complex and difficult to express (DTE) proteins

GS Xceed® Expression System toolbox now also includes GS piggyBac® a unique and versatile cell line engineering technology.  A proven transposon-based technology that preferentially targets stable regions of the genome associated with highly expressed genes.


Express vector cargos into the host cell genome with high efficiency and yields

The GS piggyBac® system uses an engineered hyperactive piggyBac™ transposase enzyme to insert GS Xceed® expression vector cargos into the host cell genome with high efficiency with the following benefits:

  • Combining our GS System® with piggyBac™ transposon technology results in increased yields versus GS System® alone with both pools and clones 1,2
  • Enhanced performance of GS piggyBac® is observed with low versus high expressing monoclonal antibodies 1,2
  • Accommodating a large DNA cargo (> 200kb) makes GS piggyBac® highly suited to support cell line engineering needed to enhance complex protein expression 3
  • Preferentially targeting genetically stable parts of the genome at high efficiency has the potential to improve cell line stability 4, 5, 6

Available to new and existing GS System® license holders. Contact us to learn more.

Combining our GS System®with piggyBac™transposon technology results in increased yields versus GS System®alone with both pools and clones

For latest research using the piggyBac™ technology

Accelerating translation of protein biologics into clinical development: current trends

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Utilising GS piggyBac™ transposon technology to enhance the performance of the Lonza GS Gene Expression System in stable CHO pool construction

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Right first time: Successfully progressing biotherapeutics from discovery to the clinic

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Tools to meet the challenge of complex protein expression

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Citations:

1 Lonza In-house data
2 Rajendra et al. (2016), Biotechnology. Progress. 32:1301-1307
3 Liet al (2013), Disease Model and Mechanisms 6:828-833
4 Rajendra et al. (2017), Biotechnology Progress 33:1436
5 Matasci et al. (2011), Biotechnol Bioeng 108:214
6 Mossine et al. (2013), PLoS One