Assess the frequency of pre-existing B cell responses

The Epibase ® in vitro B cell FluoroSpot assay is designed to identify immunoglobulin (IgA, IgE, IgG and  IgM) secreting B cells. The assay can be used to assess the frequency of pre-existing B cell responses against a biotherapeutic protein in healthy donors, as well as monitoring B cell responses to a biotherapeutic protein during clinical trials.

Recall B Cell FluoroSpot Assay

  • Detection of total and antigen-specific IgA, IgG, IgE, and IgM secreting B cells
  • Blood samples (PBMC) or suspensions of lymphoid tissues can be tested
  • Identification and enumeration of total and antigen-specific secreting cells
  • Highly sensitive, single cell analysis and quantitation of the number of circulating antigen-specific memory B cells
  • Assay removes the requirement for customized ADA (anti-drug antibody) assay development and also removes the impact of free-drug on the antibody detection