Identify HLA-binding peptides from a whole protein

The Epibase® MAPPs (MHC-associated peptide proteomics) assay identifies HLA-binding peptides naturally processed and presented from a whole biotherapeutic protein. The regions identified can be targeted for removal of potential T cell epitopes or ‘deimmunization’.

MAPPs Assay

  • Dendritic cells (DCs) are generated from healthy donors and loaded with test biotherapeutic protein
  • DCs take-up, process and present peptides from the protein via HLA Class II molecules
  • DC lysis and immunoprecipitation of HLA-DR molecules
  • Elution of peptides from HLA-DR molecules
  • Peptides identified by mass spectrometry and mapped to biotherapeutic protein sequence
  • Internal proteins included as controls
  • Immunogenicity risk of identified peptides predicted