Assess the frequency of pre-existing B cell responses

The Epibase® in vitro B cell FluoroSpot assay is designed to identify immunoglobulin (IgA, IgE, IgG and IgM) secreting B cells. The assay can be used to assess the frequency of pre-existing B cell responses against a viral protein or tumor target in healthy donors or patient samples. The assays can also be used to monitor B cell responses to a vaccine during clinical trials.

Recall B Cell FluoroSpot Assay

  • Detection of total and antigen-specific IgA, IgG, IgE, and IgM secreting B cells
  • Blood samples (PBMC) or suspensions of lymphoid tissues can be tested
  • Identification and enumeration of total and antigen-specific secreting cells
  • Highly sensitive, single cell analysis and quantitation of the number of circulating antigen-specific memory B cells
  • Assay removed the requirement for developing a customized antibody screening assay

This assay can be used to detect B cell responses towards natural infections or after vaccination (recall responses), as represented in the graph below.

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